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Equilibration

Equilibration in chromatography refers to the establishment of a steady-state distribution of analytes between the mobile and stationary phases.
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The statement of the theorem

Let Cm(t)C_{m}(t) and Cs(t)C_{s}(t) be the concentrations of an analyte in the mobile and stationary phases, respectively. Equilibration is achieved when the rate of change of the analyte distribution approaches zero, satisfying the steady-state condition:\ndCmdt=0 and dCsdt=0\frac{dC_{m}}{dt} = 0 \text{ and } \frac{dC_{s}}{dt} = 0 \nThis implies that the rate of transfer between phases, keq(Cm−Cs/Keq)k_{eq}(C_{m} - C_{s}/K_{eq}), balances the flow dynamics.