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Buffer System pH

The pH of the buffer system significantly impacts analyte charge and therefore electrophoretic mobility, requiring careful control for optimal separation.

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The statement of the theorem

Let

H^+

be the concentration of protons and

A

be the analyte. The net charge

q

of the analyte, which depends on the buffer

\text{pH}

, is modeled by the Henderson-Hasselbalch relationship applied to the dissociation constant

\text{p}K_a

:\n\n

q = q_{max} + (q_{min} - q_{max}) \frac{1}{1 + 10^{(\text{pH} - \text{p}K_a)}}

Source: Wikipedia