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Lineweaver-Burk Plot

A double reciprocal plot (1/v vs. 1/[S]) used to determine Km and Vmax from experimental data.

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The statement of the theorem

Consider the double reciprocal plot of the initial velocity

v

versus substrate concentration

[S]

. The relationship is linear in the form

y = m x + b

, where

y = 1/v

and

x = 1/[S]

. The equation is derived from the Michaelis-Menten kinetics as:

Source: Wikipedia