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Retention Time

Retention time is the time it takes for a component to pass through the chromatographic column, a key parameter in quantification.

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The statement of the theorem

Define the retention time

t_R

for an analyte

i

as the time required for the analyte to pass the detector, measured from the injection point. It is related to the dead time

t_M

(void time) and the retention factor

k'_i

by:\n

t_R = t_M (1 + k'_i)

\nwhere

k'_i

is the retention factor, defined as

k'_i = \frac{V_{i, eq}}{V_M}

, with

V_{i, eq}

being the equilibrium volume and

V_M

being the mobile phase volume.

Source: Wikipedia